FastQCFastQC Report
Fri 1 Jun 2018
HVT5JBGX5_n01_03_12_GR_Dam_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVT5JBGX5_n01_03_12_GR_Dam_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20670554
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGGATC2308650.066.7422311
AGGATCA688150.066.6856412
AGGATCT806600.066.6598512
GGTCGCG2291250.066.6563951
GGATCTT316250.065.84717613
AGGATCG268650.065.8407712
GGATCCA205050.065.6447813
CGGCCGA2373350.065.6438756
CCGAGGA2353100.065.605419
CGAGGAT2351650.065.5886710
GGATCTG187100.065.3020413
GTCGCGG2348550.065.267932
GGATCAT221800.065.2002813
GGATCTA105350.065.146913
GGCCGAG2393650.065.13367
GGATCAC127250.065.12903613
GGATCTC214700.065.0090513
GGATCAA212750.064.9468813
GGATCCG108600.064.74419413
CGCGGCC2389150.064.733754
Produced by FastQC (version 0.11.5)