FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67402389
Sequences flagged as poor quality0
Sequence length76
%GC63

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG725549610.764449313510237No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA37650.019.53976870
GTGTAGA2466650.018.73704770
AGTGTAG3268200.018.08626270
TGTAGAT1866100.017.61081170
GAGTGTA4167800.015.86854970
TAGATCT1012250.015.72936670
GTAGATC1394450.015.66887870
AGTGTAT22700.013.7350270
AGATCTC866450.013.51226270
CGAGTGT5112100.012.97616269
TATCATT67650.012.63102250
TCATTAA74550.011.83862752
GTACTAG9850.011.717341
GTATCAT72050.011.61657449
TCTCGGT646800.011.39549269
ACGAGTG5980600.011.03743568
TCGGTGG490550.010.96915270
GTATTAG7450.010.7974921
TATATAG15950.010.5276225
TTAAAAA95750.010.42437455
Produced by FastQC (version 0.11.5)