FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n02_ATAC_MP-1000_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_ATAC_MP-1000_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52514010
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1160870.22105910403718929No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGATA32150.022.77349570
GTGTAGA2377550.017.9495470
TGTAGAT1793300.016.87230370
AGTGTAG3030450.016.70533470
ATTAAAA28800.015.80868754
AGTGTAT16650.015.78015970
CATTAAA30450.014.837213553
GAGTGTA3800200.014.78086470
CCTTATA14050.014.6880642
GTAGATC1409300.014.13905970
TTAAAAA33200.013.92452455
ACACATA81400.013.85784970
TAGATCT1035400.013.62165770
TCGGTGG511800.013.26532370
CGAGTGT4601600.012.19050169
AGATCTC936100.011.75465970
ATCATTA39350.011.48099651
TCTCGGT755400.011.23658169
TCATTAA40700.011.18669452
TATATAG11900.011.1706945
Produced by FastQC (version 0.11.5)