FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n02_9_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_9_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647777
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1319200.30223761452960135No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA92750.023.8243648
GTATCAT111000.019.9704849
ATCATTA111700.019.72074151
TCATTAA115600.019.29846652
AGTGTAG1599200.017.88418670
GTGTAGA1240200.017.75343970
CATTAAA125850.017.64321753
AGGGGGG112950.017.33606766
TATCATT131050.017.26254350
TGTAGAT927600.016.60976270
CCGTATC133200.016.5366247
GCCGTAT141500.015.78749346
GAGTGTA2036800.015.77899370
AAGGGGG133100.014.65816965
ATTAAAA160650.014.14821554
AAAGGGG139750.013.91040464
GTAGATC740600.013.81709670
TAGATCT557350.013.40700470
CGAGTGT2507600.013.16855370
AAAAGGG160600.012.27885763
Produced by FastQC (version 0.11.5)