FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n02_8_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_8_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40446539
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1538200.38030448044021764No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG1336750.018.79785370
GTGTAGA1024950.018.29583270
TGTAGAT762450.017.35834170
GAGTGTA1749600.017.03716770
CGAGTGT2210450.013.97801470
TAGATCT458300.013.7129370
GTAGATC616600.013.24898570
ACGAGTG2619200.011.73236269
GTCCTAA43000.011.7122071
AGATCTC442650.011.44367470
TATACCG42400.011.1383735
GTCCTAC66550.010.4580271
TCTATAC40800.010.3738343
CATACAC153350.010.1733773
TACACCG92850.010.0596585
TAGGACG57700.09.9427444
GACGAGT3108750.09.92213268
GTATTAG23950.09.6379171
TCATACA157550.09.63513852
GTGTATG141800.09.619071
Produced by FastQC (version 0.11.5)