FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n02_6_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n02_6_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38367133
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3985911.0388865907702824No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG94700.022.26580666
CGTATCA112900.020.75012848
AAGGGGG101600.020.58053265
AAAGGGG120250.017.47586864
AGTGTAG1170450.017.06844970
CCGTATC139750.016.71318447
GCCGTAT140800.016.71143746
GTGTAGA931250.016.28790370
GTATCAT143900.016.1096849
TCATTAA153100.015.7834252
CATTAAA155350.015.57714153
GAGTGTA1485600.015.47783670
ATCATTA159850.014.89706751
TGTAGAT731650.014.47365870
CTAGTAC43600.013.799373
CGAGTGT1841150.013.23279270
CGCCGTA182150.013.06855745
AAAAGGG163850.012.99658263
TATCATT183600.012.93146950
CCTAGTA47100.011.36222652
Produced by FastQC (version 0.11.5)