FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n01_ATAC_MP-1000_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_ATAC_MP-1000_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38420177
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGT5421381.4110762685970968No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3268120.8506259614576998No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG781990.20353628251113992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG623800.062.1094270
AAGGGGG725700.053.36056569
AAAGGGG742550.052.2346768
CGTATGC795900.050.17871541
GTATGCC795850.050.1175342
AAAAGGG778350.049.95064567
TCGTATG805150.049.64830840
CTCGTAT810250.048.68275539
TGAAAAA848550.047.27710359
TATGCCG850400.046.94524443
TCTCGTA841050.046.77708438
GAAAAAA862900.046.55550460
AAAAAGG840600.046.43387266
CCGTCTT866050.045.97728747
AAAAAAG854300.045.91510465
TTGAAAA880900.045.4255158
CTTGAAA885150.045.14842257
GCCGTCT894000.044.58631546
TCTGCTT903550.044.1503453
TTCTGCT909950.043.82753452
Produced by FastQC (version 0.11.5)