FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n01_ATAC_MP-1000_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_ATAC_MP-1000_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52514010
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3659130.6967911991485701No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT585070.11141217362757101No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG109250.037.10028570
TCGTATG116950.035.58132640
AAAAAAG116450.035.2955365
AAAGGGG117100.034.82708468
GAAAAAA124150.034.1819760
AAAAGGG121250.033.63640667
TGAAAAA135700.030.9888659
AAGGGGG131500.030.93024369
CGTATGC137750.030.61593441
CTCGTAT138550.029.9325239
TTGAAAA140850.029.7562458
GTATGCC141300.029.64969442
AAAAAGG142250.028.72123166
CTTGAAA188900.022.40975857
TGTATAA14450.020.58226614
GTGTATA16200.020.51889613
TCTCGTA212200.019.62532638
CCGTCTT227050.018.63807947
TCTGCTT240300.017.4820453
CAGGCAG2577750.016.4828570
Produced by FastQC (version 0.11.5)