FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n01_9_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_9_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43647777
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4073070.9331677991298389No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT789350.18084540708682598No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG146250.038.60345570
TCGTATG165400.035.0608940
CGTATGC175700.033.0865341
CTCGTAT178950.032.0337839
GTATGCC192000.030.29682242
AAGGGGG199200.028.30752269
TCTCGTA214000.026.76963638
AAAGGGG212100.026.6550768
AAAAGGG224850.025.16047367
CCGTCTT246800.023.51443547
TATGCCG266600.021.92489843
GCCGTCT289400.020.07704446
AAAAAAG299200.019.22527965
GAAAAAA317850.018.76000460
AAAAAGG304450.018.7554666
TGCTTGA338750.017.60264255
GCTTGAA338800.017.41415856
ACGCTAC1659650.017.31889270
CTTGAAA347950.016.96638157
TTGAAAA349400.016.84578758
Produced by FastQC (version 0.11.5)