FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n01_8_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_8_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40446539
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2475290.6119905586977418No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGT765150.18917564244495677No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG122450.045.8783270
TCGTATG140950.040.5717240
CGTATGC150200.038.79659741
GTATGCC156150.037.43219442
CTCGTAT152500.036.87896739
AAGGGGG152750.036.75573369
AAAGGGG156700.036.03435568
AAAAGGG185050.030.55290867
TCTCGTA189450.029.81387938
CCGTCTT226700.025.70727747
AAAAAGG223550.025.54278866
TATGCCG244500.024.17875543
AAAAAAG252900.022.92484565
GAAAAAA267550.022.69284660
TTGAAAA263150.022.52677358
CTTGAAA267150.022.04552857
GCCGTCT274400.021.3275946
TGAAAAA285300.021.04787659
TCTGCTT285400.020.46038853
TGCTTGA301650.019.47724755
Produced by FastQC (version 0.11.5)