FastQCFastQC Report
Sat 16 Jun 2018
HVJJYBGX5_n01_6_MP-2.5hpc_1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJJYBGX5_n01_6_MP-2.5hpc_1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38367133
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT2966030.773065321299874No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2653160.6915189623368522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG343050.060.95493770
AAGGGGG396600.052.71732369
TCGTATG438000.049.39673240
CGTATGC440600.049.2259341
GTATGCC443700.048.8288942
AAAGGGG430950.048.6259568
CTCGTAT441300.047.99517439
AAAAGGG476700.044.13706667
TCTCGTA480650.043.85949738
CCGTCTT501750.043.1818247
TATGCCG506300.042.93798443
GCCGTCT523200.041.35066646
AAAAAGG548350.038.5382166
TCTGCTT580550.037.49028853
TGCTTGA584000.037.25714555
TTCTGCT591200.036.8380452
GCTTGAA593900.036.6371456
CTTGAAA601550.036.24121557
TCTTCTG614700.035.50746550
CTGCTTG616500.035.34893454
Produced by FastQC (version 0.11.5)