FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15758818
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5187973.2921060450092132No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA333310.21150697977475214No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG38450.047.3978115
GCACACG41850.046.30638511
CACACGT43200.043.96832312
ACACGTC43300.042.41317413
CACGTCT42650.042.1573614
CGTCTGA43850.041.88052716
AGCACAC47550.041.41742310
AGAGCAC54150.038.4357578
TCGGAAG72650.034.192613
CTGAACT49250.033.59851519
ATCGGAA79300.031.6386622
CGGAAGA78100.031.580164
GATCGGA82100.030.7308521
GAACTCC53300.029.07868621
ACGTCCG18250.027.61668632
CCGCACA21100.027.36947836
TGAACTC63600.026.45868720
TCCGCAC21900.026.21011435
CAGTCAC45050.025.2492327
CGCACAT23200.024.43917737
Produced by FastQC (version 0.11.5)