FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13733378
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC201080.14641699951752585TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG151080.11000935094046055No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29650.045.44718646
CGTATGC34900.038.91341844
GTATGCC35750.037.98737345
CTAATCG38450.034.8705533
TCGTATG40900.033.6331143
ATGCCGT43000.031.5002147
ACGTCTG46500.029.80317715
TGCCGTC46150.029.1979348
CTCGTAT46900.028.73404542
GCACACG49650.028.12379811
GCCGTCT49350.027.80028749
ACTAATC49150.027.77827832
CACACGT52500.026.46375812
CACTAAT55100.024.71527531
ACACGTC57600.024.12052513
AGTCACT63200.021.76755728
CACGTCT64500.021.5402714
GTCACTA65450.020.80583829
CGTCTGA67600.020.5007816
TCTCGTA66000.020.20666741
Produced by FastQC (version 0.11.5)