FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8100541
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC397180.490312930951155TruSeq Adapter, Index 3 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG87950.10857299530981943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG47600.059.77781346
CGTATGC50050.056.99535444
GTATGCC51200.055.78389445
ATGCCGT53300.053.4507347
TCGTATG54850.052.1990543
ACGTCTG55600.051.73747315
GTCACGT55900.051.16416529
GCACACG56800.051.0144111
CACGTAG56550.050.6414231
TGCCGTC57300.049.53589248
AGTCACG58550.048.6675828
TCACGTA59100.048.39415430
CTCGTAT58750.048.25909442
CACACGT60550.047.91246812
GCCGTCT59700.047.778749
ACACGTC62450.046.2866513
CACGTCT64950.044.5050314
ACGTAGA65900.043.8284832
CGTAGAG65850.043.7011833
CGTCTGA67600.042.81188616
Produced by FastQC (version 0.11.5)