FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Tsu-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11462329
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC426910.37244612329658305TruSeq Adapter, Index 8 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG133160.11617185303266028No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54600.057.1159546
CGTATGC57550.054.43313244
GTATGCC58150.053.93167545
ATGCCGT60250.050.88825247
TCGTATG60850.050.5614643
ACGTCTG70050.049.05795315
GCACACG71900.047.69812811
TGCCGTC65300.047.11379648
CACACGT74200.046.3137412
ACACGTC76800.044.56356413
GCCGTCT67250.044.49761649
CTCGTAT69800.043.93073342
CGTCTGA84100.041.0702416
CACGTCT85950.040.14534814
AGCACAC90400.038.36255310
GAGCACA93150.037.3051389
TCTCGTA84000.036.67103241
TCGGAAG96800.035.6125373
ATCTCGT92500.033.75625240
ATCGGAA103350.033.288962
Produced by FastQC (version 0.11.5)