FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14746807
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC425470.2885166938171768TruSeq Adapter, Index 23 (97% over 38bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG170770.11580133923228261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG55450.057.62982646
CGTATGC63400.050.4067944
GTATGCC63900.050.06647545
ATGCCGT66450.048.19541547
TCGTATG69400.046.35257743
TGCCGTC74300.043.29127548
ACGTCTG79000.042.12803315
CTCGTAT74250.041.7703842
GCCGTCT81850.039.25324649
ACTAATC78650.039.03565236
CACACGT88000.037.85899412
GCACACG87950.037.72122611
CCAACTA85900.037.4528133
ACACGTC91850.036.1956413
AACTAAT88800.036.15072335
CAACTAA97900.033.07650434
CGTCTGA100750.032.9643716
CACGTCT101600.032.6192514
TCTCGTA96000.032.4525241
CTAATCT97800.031.42764337
Produced by FastQC (version 0.11.5)