FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10338108
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC395920.38297142958847014TruSeq Adapter, Index 16 (98% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG103740.10034718151522504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49900.060.74015846
CCCGTCC53200.057.51599533
CACCCGT55350.055.5380931
CGTATGC55050.055.18979344
ACCCGTC58050.052.89281532
ATGCCGT58000.052.43800447
GTATGCC58650.052.1000845
GTCACCC60250.051.13529229
TCACCCG61150.050.4984230
TCGTATG60350.050.11291543
ACGTCTG64100.048.971415
GCACACG65150.048.34329211
TGCCGTC62800.048.15088348
CACACGT68100.046.3002912
GCCGTCT65550.045.64852549
ACACGTC70050.044.8115913
CTCGTAT66400.044.0211142
CCGTCCA69800.043.83719334
CGTCTGA76050.041.32257516
CACGTCT78300.040.49230214
Produced by FastQC (version 0.11.5)