FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Stw-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13007051
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC543030.41748894503450473TruSeq Adapter, Index 7 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG142930.10988655307033086No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG66400.059.8808146
CGTATGC71900.055.25366644
GTATGCC74200.053.7290245
ATGCCGT74900.052.71141447
TCGTATG76850.051.05765543
GCACACG83800.049.9870411
ACGTCTG84100.049.89098715
TGCCGTC82900.047.66633648
CACACGT89850.046.73803712
ACACGTC91250.045.9055513
CTCGTAT84400.045.8712142
GCCGTCT86800.044.75644749
CGTCTGA101750.041.30546616
CACGTCT103800.040.7260114
AGCACAC108300.039.03409210
GAGCACA111100.038.3336759
TCGGAAG113350.037.1120763
TCTCGTA104450.036.99926841
CATCTCG108700.035.9085539
CAGTCAC120500.035.09504327
Produced by FastQC (version 0.11.5)