FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12695835
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC446380.35159562171373526TruSeq Adapter, Index 7 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG141820.11170592560473573No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG54500.056.25927746
CGTATGC59300.051.53056344
GTATGCC60200.050.81731445
ATGCCGT63450.048.48867847
TCGTATG65050.047.24482743
TGCCGTC68550.045.136548
CTCGTAT72600.041.94723542
ACGTCTG74900.041.7233515
GCCGTCT75600.041.20269849
GCACACG80650.039.0085411
CACACGT83150.037.96226512
ACACGTC83750.037.35557613
CGTCTGA91000.034.3034816
TCTCGTA91100.033.65874541
CACGTCT95400.032.9042414
AGCACAC98400.032.39883410
CAGTCAC103150.030.33981927
GATCTCG104600.029.28325539
GAGCACA110550.029.122719
CTGAACT110450.028.92911319
Produced by FastQC (version 0.11.5)