FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12604307
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG138790.110113154178171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGCC2556.45046E-613.7298791
CGCCCCT5206.002665E-1112.119261
CGGGCCT8100.011.6703971
GCCGAAT16650.011.5652591
GCCGGTT31000.011.5198131
TATGCCG11400.011.05265648
CCCCCCT4207.861163E-710.8367971
GCGGGCA9350.010.4846351
GGCGAAT31250.010.3072951
CTCGGTT45800.010.0905631
GCCGGAT24100.09.8786761
GCGGGCT7151.4915713E-109.7933411
GGGGAAT28450.09.3527251
GCGGGGT5653.6417077E-79.2950061
CCCGCCT18050.09.11648751
GCGGGTT30100.09.0726671
GGGGACT12400.09.0351461
GGCAGCT55150.09.01466751
GCGGGAT19850.08.9953191
GCGGGGA9001.6370905E-118.9473051
Produced by FastQC (version 0.11.5)