FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.St-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8111200
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC231870.2858639905316106TruSeq Adapter, Index 6 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82870.10216737350823553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG30850.053.09738546
GTCACGC31550.052.5917529
GTATGCC33400.049.14924245
CGTATGC33500.048.8980544
TCACGCC34950.047.47582630
ATGCCGT36000.045.98734747
TCGTATG36350.045.1610743
CACGCCA36600.044.95516231
TGCCGTC38900.042.55872348
ACGTCTG39050.042.47859215
GCACACG40100.041.45409411
TATCTCG39750.041.3885939
CTCGTAT40350.040.42514842
CACACGT42050.039.61495212
GCCGTCT41900.039.42681549
ACGCCAA41950.039.30486732
ACACGTC42800.038.92052513
CGCCAAT43900.037.71800233
AGTCACG45250.036.82253328
CGTCTGA51050.032.76762416
Produced by FastQC (version 0.11.5)