FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12001040
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT647660.5396698952757428TruSeq Adapter, Index 25 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG149310.12441421743448901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG79000.062.6012248
GTATGCC86600.057.27020647
CGTATGC86650.057.15685346
ATGCCGT87850.056.33321449
TCGTATG92600.053.4477845
TGCCGTC92950.053.35415650
TATCTCG93800.052.46942541
CTCGTAT95200.051.73092344
ACGTCTG101250.049.49418615
CACACTG101200.049.12394331
GCCGTCT100950.048.95244651
GCACACG106700.047.26111611
ACACGTC108500.046.380313
CACACGT109750.045.7882812
TCTCGTA110050.044.87783443
CACGTCT114000.044.29632614
TGATATA112600.044.2422736
ACACTGA113800.043.56167232
ACTGATA114650.043.4201234
CGTCTGA115750.043.41480316
Produced by FastQC (version 0.11.5)