FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9806971
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG106690.1087899617527165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG13450.040.33441548
CGTATGC18350.030.5186846
GTATGCC18950.028.99754347
ATGCCGT20900.026.12363249
ACGTCTG24600.022.76077315
GCACACG25150.022.12399111
TCGTATG25900.021.21797445
TCCGTAT26300.021.0317937
TATCTCG27200.020.20542341
TGCCGTC27700.020.08904650
GTTCCGT28900.019.01871335
CGTATCT28900.019.01735339
CTCGTAT28200.018.99073844
CACACGT30100.018.36921112
ACACGTC32150.017.4155313
GCCGTCT31950.017.3070851
CCGTATC32000.017.28488338
AGTTCCG34350.016.81670834
TTCCGTA35550.015.75633636
CGTCTGA37500.015.21117916
Produced by FastQC (version 0.11.5)