FastQCFastQC Report
Thu 12 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Shahdara_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10325953
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC260740.2525093809743275TruSeq Adapter, Index 5 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG114280.11067259360951961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36850.055.46848746
CGTATGC40050.051.1259244
GTATGCC41750.049.37829645
ATGCCGT43700.046.6934547
TCGTATG45600.044.75092743
ACGTCTG48550.043.89774715
GCACACG50450.042.17555611
TGCCGTC48650.041.8701848
CACACGT51950.041.42913412
ACACGTC56250.038.26210413
GCCGTCT53900.037.85574749
CTCGTAT48400.037.24541542
CACACAG61250.034.52404831
CGTCTGA63400.033.61586816
CACGTCT64300.033.58059314
CACAGTG63150.033.31835633
GAGCACA68000.032.4742289
ACACAGT68000.030.99330932
AGCACAC70600.030.43561210
TCACACA73900.028.75546530
Produced by FastQC (version 0.11.5)