FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14742449
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC1123740.7622478463381491TruSeq Adapter, Index 22 (97% over 37bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG157080.10654946135475864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG125800.063.65637646
CGTATGC133600.060.04877544
GTATGCC134950.059.5248745
ATGCCGT138500.057.71762547
TCGTATG140550.056.95647443
TGCCGTC144550.055.22889348
CTCGTAT144300.055.01897442
ACGGTAG151050.053.65482332
ACGTCTG154300.053.07067515
GCCGTCT152450.052.20426649
GTCACGG157550.051.5524829
AGTCACG158400.051.22989728
GGTAGCA162250.049.90656334
GCACACG165600.049.5764311
ACACGTC166900.049.21124613
CACACGT167900.048.9598312
CGGTAGC170350.047.65765833
CACGTCT173650.047.2978614
TCTCGTA167200.047.25366241
CGTCTGA176800.046.53449616
Produced by FastQC (version 0.11.5)