FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8003502
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT131280.16402819665691343TruSeq Adapter, Index 13 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG84320.10535388133844409No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG17400.052.0983348
GTATGCC23050.039.17642647
CGTATGC23900.037.9295746
ATGCCGT25700.034.9995549
TGCCGTC26300.034.46551550
TCGTATG27150.033.64871245
ACGTCTG27850.033.17254315
GCACACG29300.031.65033711
GCCGTCT30100.030.81182351
CTCGTAT30950.029.29172744
CACACGT32450.028.90148412
ACACGTC33200.028.45939813
CACGTCT37400.025.07644514
AGCACAC39900.024.73297110
CGTCTGA39750.023.68167716
ACAGTCA39350.023.48905432
CACAGTC40400.023.05189331
GAGCACA44700.022.5466659
TCTCGTA40950.022.05320443
GTCACAG46200.020.45939629
Produced by FastQC (version 0.11.5)