FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Oy-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6545295
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC186600.28509028240896706TruSeq Adapter, Index 4 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG73060.11162216523472204No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG23300.056.9332346
CGTATGC26150.050.8640644
GTATGCC26100.050.69212345
ATGCCGT28450.046.7495347
TCGTATG29650.045.0966743
ACGTCTG30700.044.68385315
GCACACG31050.044.0671311
ACTGACC32450.041.75625632
TGCCGTC32550.040.64589748
CTCGTAT31850.040.5557642
CACACGT34500.039.96471412
GCCGTCT33350.039.56381649
CACTGAC35300.038.4850731
ACACGTC37550.036.62538513
CGTCTGA41250.033.76437416
CACGTCT40950.033.67016614
AGCACAC41700.033.65125710
AGTCACT40850.033.3369928
GAGCACA46750.030.0910559
TCTCGTA43850.029.9371641
Produced by FastQC (version 0.11.5)