FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14670634
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT2048821.396544961860544TruSeq Adapter, Index 22 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182530.12441861749124135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG227550.065.1550248
CGTACGT228550.065.0101934
GTACGTA231900.064.22246635
CGTATGC230950.064.18198446
CCGTACG233000.063.9342933
GTATGCC233000.063.7069947
TACGTAA235150.063.2606236
ATGCCGT238150.062.42794849
TCGTATG240200.061.61193545
TGCCGTC242850.061.08962250
ACGTAAT245750.060.4031537
ACCGTAC247300.060.2098332
CTCGTAT242850.060.10445844
GCCGTCT252900.058.53663651
ACGTCTG271100.055.67472515
TCTCGTA264350.055.05774343
CGTAATC268050.054.51431338
CACCGTA282800.052.80076631
CGTCTGA287250.052.64199416
AATCTCG278000.052.49501841
Produced by FastQC (version 0.11.5)