FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11038568
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG122040.1105578187315601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG11950.024.89552146
CGTATGC16150.018.85560644
GTATGCC17800.017.3042445
ATGCCGT19150.015.71795147
GCGGGGT4750.014.0044461
TCGTATG21800.013.48716143
TGCCGTC23100.013.030189548
ACGTCTG25300.012.44907515
CGGGCCT7900.012.4090041
GCACACG25800.011.93631311
CGGGCAT16050.011.7794421
GCGGGAT18850.011.5155921
CTCGTAT28050.010.85689742
CGCGCAT5552.2828317E-910.7241261
GCGGTAT13400.010.7123561
GTCGGAT33750.010.6848751
GCCGTCT28050.010.60572849
GCGGGTA11250.010.5811381
GGCGTAT12650.010.5171731
CGGGGAT18800.010.42884351
Produced by FastQC (version 0.11.5)