FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.N13_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5862651
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC333900.5695375692668726TruSeq Adapter, Index 3 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37300.061.65075346
CGTATGC39250.058.23313544
GTATGCC40200.057.46647345
ATGCCGT41550.055.25953747
TCGTATG41900.054.21644643
GCACACG45250.051.97068811
TGCCGTC44750.051.6204348
ACGTCTG45800.051.4998415
CACACGT46550.050.66966212
CTCGTAT44650.050.17299742
ACTTAGG47050.049.77882832
CACTTAG47950.048.9905331
GCCGTCT47000.048.47530449
TTAGGCA49450.048.07001534
ACACGTC49650.047.7879313
TCACTTA50100.046.88693630
TAGGCAT51300.045.58621635
CACGTCT53550.044.1772514
AGTCACT53550.043.86246528
CGTCTGA53650.043.7035316
Produced by FastQC (version 0.11.5)