FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9895503
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT476450.4814813355116966TruSeq Adapter, Index 20 (97% over 44bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG109610.11076748700899794No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG57500.060.0151948
CGTATGC61150.056.8922346
GTATGCC61700.056.2146347
CGTGGCC63100.054.8719933
ATGCCGT64550.053.6754549
ACGTGGC66550.052.2930632
TCGTATG67500.051.59486845
GCCTTAT68400.050.92731537
TGCCGTC68100.050.722750
CACGTGG69250.050.30475631
TATCTCG69550.049.82952541
TCACGTG71400.048.8850830
GTCACGT71350.048.86854629
CTCGTAT72050.047.9968144
GCCGTCT72450.047.7738551
GTGGCCT75850.045.97114634
GGCCTTA77000.045.46689236
ACGTCTG79250.044.86402515
AGTCACG77900.044.8033828
ACACGTC88350.040.4413113
Produced by FastQC (version 0.11.5)