FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8306115
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG99490.11977922289782889No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG8600.024.41847646
CGTATGC12150.016.70853644
GTATGCC13200.015.11410345
GCCGGAT17500.013.8053291
ATGCCGT15900.013.42752247
GCGGGCT5650.013.0138741
ACGTCTG18200.012.3062215
CGCCCTT11600.012.07362651
CGGGGCT10500.012.0046351
GGCGAAT21300.011.9999391
CGGGCCT5853.6379788E-1211.9704331
GGGCGCT2952.8847819E-511.8689891
TCGTATG17900.011.73234743
CGCGCTA3003.425726E-511.6711721
TATCTCG19600.010.89411339
TGCCGTC19100.010.81133248
GGGGAAT17900.010.56273651
CTCGTAT20900.010.04864142
CCCCGCT6704.7293724E-109.9292071
CCGGGAT16650.09.8836961
Produced by FastQC (version 0.11.5)