FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mt-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6670367
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC383790.5753656432996865TruSeq Adapter, Index 2 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71240.10680072026021956No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG45250.061.3383846
GTATGCC47100.059.0074145
CGTATGC48650.057.12827744
ATGCCGT50050.055.38501447
ACGTCTG53050.054.62154815
TCGTATG50650.054.18179343
TATCTCG51700.052.94994439
GCACACG55550.052.41572611
CCGATGT54900.051.7780933
TGCCGTC53750.051.3116148
GCCGTCT53900.050.64667549
CACACGT57600.050.61023312
CTCGTAT54150.050.42305442
ACACGTC59100.049.5033513
GATGTAT60800.046.7532235
ACCGATG62800.045.20953832
CGTCTGA64600.045.12615216
CGATGTA63550.044.89532534
CACGTCT64850.044.79029514
AGCACAC66250.044.4251810
Produced by FastQC (version 0.11.5)