FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17848550
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT9385765.258556017155455TruSeq Adapter, Index 18 (97% over 40bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233500.13082295200450456No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197750.11079331374257292No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCGC1014250.067.87334433
TATGCCG1016300.067.70232448
GTCCGCA1020050.067.42145534
CGTATGC1024100.067.3283146
GTATGCC1023550.067.3166147
CCGCACA1018000.067.2819236
ACGTCCG1025100.067.2524632
TCCGCAC1024050.067.1265835
ATGCCGT1025300.067.0610349
TCGTATG1028300.066.99126445
CGCACAT1019500.066.9347537
TGCCGTC1032000.066.5938350
CTCGTAT1025000.066.4359444
GTCACGT1044250.066.11639429
AGTCACG1045850.066.06341628
ACGTCTG1054850.066.03950515
GCCGTCT1040750.065.9661351
TCACGTC1051100.065.663330
GCACACG1073750.064.9985911
GCACATC1038900.064.9845838
Produced by FastQC (version 0.11.5)