FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7742749
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGC299190.38641314602862625TruSeq Adapter, Index 9 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82860.1070162548211236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG36150.061.0962346
GTATGCC40300.054.97909545
CGTATGC41250.054.2223944
ATGCCGT41250.053.45662347
TCGTATG44300.050.25317843
ACGTCTG46650.048.0862815
TGCCGTC47150.046.8401848
GCACACG48000.046.7335511
ACACGTC50700.044.58990513
AGTCACG50200.044.41952528
CACACGT50550.044.23732812
GCCGTCT50700.043.5584749
CTCGTAT49800.043.3690642
GTCACGA51450.043.34117529
CGATCAG52300.042.37123533
AGCACAC56750.039.83626610
CGTCTGA58400.038.41138516
TCACGAT58300.038.24926830
CACGTCT59150.038.2204114
TCTCGTA57750.037.39901441
Produced by FastQC (version 0.11.5)