FastQCFastQC Report
Wed 11 Jul 2018
HVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVFY7BGX5_n01_NutriNet.AtRep.Mr-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9482815
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC441340.46541032383316555TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG97740.10307065992534917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG52050.062.60254346
CGTATGC58150.056.5819444
GTATGCC58150.056.3405745
ATGCCGT60300.054.15446547
TCGTATG61750.053.2843643
ACGTCTG63550.051.76420215
TGCCGTC67500.048.68884348
CTCGTAT66050.048.5980842
GCCGTCT69200.047.3905749
GCACACG69800.047.2296811
ACACGTC70450.046.545313
CACACGT72700.045.3454512
CACGTCT76050.043.30196814
TCACGAT76050.042.90297335
AGCACAC78150.042.0933810
TCGGAAG81500.040.4069563
CGTCTGA82600.040.12263516
TCTCGTA80150.040.09279341
GAGCACA85350.039.0750669
GATCTCG83950.038.0294139
Produced by FastQC (version 0.11.5)