FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13386197
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG655640.4897881003842988No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA181910.1358937120079736No Hit
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG144730.10811883315328469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG29100.053.02166415
GCACACG29550.052.33223311
CACACGT29550.052.33223312
ACACGTC30700.049.68737813
CGTCTGA31950.047.52726716
CACGTCT31900.047.2697114
AGCACAC34900.045.41303310
CTGAACT35900.042.78668619
CGGGGGG20900.041.4323581
GAACTCC38850.039.90054721
AGAGCAC42050.038.192138
CAGTCAC27150.036.73390627
AGGGGGG28000.035.5967981
CCAGTCA32950.035.57799526
TGAACTC44000.035.388120
CTCCAGT41600.033.47859624
TGGGGGG24700.029.6205581
TCGGAAG63200.029.0696093
AACTCCA53650.028.56845322
CGGAAGA65950.027.7515414
Produced by FastQC (version 0.11.5)