FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Mh-1_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Mh-1_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22482820
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATCGATCTCGTATGC844240.3755044963220806TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG111100.057.56749346
CGTATGC118000.054.2344344
GTATGCC121500.052.745
CTAATCG127350.050.2430533
ATGCCGT128650.049.93168647
TCGTATG128950.049.81895443
GCACACG130900.049.45773711
ACGTCTG133600.048.53939415
TGCCGTC135950.047.25012648
CACACGT139550.046.54287312
ACACGTC141250.046.00789613
CTCGTAT143100.044.4024842
GCCGTCT148550.043.45271749
ACTAATC148250.043.3009732
CACTAAT151650.042.6529131
CACGTCT159500.040.83179514
AGCACAC164250.039.7352210
CGTCTGA164700.039.62973816
TCTCGTA161900.038.98607341
GTCACTA168700.038.30265829
Produced by FastQC (version 0.11.5)