FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Mh-1_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Mh-1_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12823339
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGC552910.4311747509755454TruSeq Adapter, Index 8 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG67150.061.41372346
CGTATGC74850.054.82109544
GTATGCC76550.054.0597545
ATGCCGT75900.052.8114647
TCGTATG77750.051.7858443
TGCCGTC78200.050.45178248
ACGTCTG92200.049.38256515
GCACACG93200.048.8492811
GCCGTCT81150.048.14039249
CTCGTAT84700.047.78367242
CACACGT97450.046.6472212
ACACGTC98500.046.14978413
CACGTCT109450.041.5008914
AGCACAC111500.041.2398710
CGTCTGA111050.040.8431116
TCGGAAG114550.040.1112673
TCTCGTA104850.038.93334241
GAGCACA119050.038.9184349
GATCGGA122000.037.3501171
ATCGGAA125200.036.533682
Produced by FastQC (version 0.11.5)