FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344032
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAACTAATCTCGTATGC308780.27219598816364415TruSeq Adapter, Index 23 (97% over 38bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG38150.057.71690846
CGTATGC43650.050.44733444
GTATGCC44850.049.0971445
ATGCCGT46250.047.7598147
TCGTATG48950.045.19976443
TGCCGTC53400.041.1679848
CTCGTAT54250.040.52527642
ACGTCTG58000.039.10003315
GCCGTCT58400.037.70127549
GCACACG60050.037.6471811
AACTAAT59500.037.29678335
ACTAATC61400.035.7450136
CACACGT63850.035.62586212
ACACGTC64150.035.4043913
CCAACTA63200.035.22248533
CACGTCT72800.031.48666414
CAACTAA72450.030.96648434
CGTCTGA74350.030.64386616
CTAATCT73650.030.22752437
TCTCGTA73000.030.16340841
Produced by FastQC (version 0.11.5)