FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13416503
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCATCTCGTATGC1203410.8969624946232263TruSeq Adapter, Index 16 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG140550.064.3345646
CCCGTCC146500.062.54982433
CGTATGC147700.061.32048444
GTATGCC148250.061.02054645
CACCCGT152900.060.1118731
ACCCGTC152950.060.0253632
ATGCCGT151350.059.4895847
TCACCCG155550.059.10897430
TCGTATG152500.058.70145843
GTCACCC158650.058.0915529
TGCCGTC159100.056.5917548
CTCGTAT157400.055.47130242
ACGTCTG167950.055.34570315
GCCGTCT163750.054.38389649
CCGTCCA173100.052.91649234
GCACACG176500.052.7995111
CACACGT176800.052.57175412
ACACGTC179400.051.69280613
CGTCTGA185900.049.88982416
TCTCGTA173000.049.6180541
Produced by FastQC (version 0.11.5)