FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Kn-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12567061
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC393620.31321563570034394TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG49800.057.00898446
CGTATGC57100.049.96923444
GTATGCC58100.049.40947745
ATGCCGT57050.049.1503947
TCGTATG61100.046.35413743
ACGTCTG68050.044.07340615
TGCCGTC65250.043.34918648
GCACACG70300.042.8607411
CACACGT72700.041.34969312
GCCGTCT69300.040.7129849
CTCGTAT70550.039.94598442
ACACGTC77300.038.93400213
CACGTCT84500.035.49202314
CGTCTGA85700.035.20160316
AGCACAC89550.034.1163210
GAGCACA91350.033.941839
TCTCGTA87600.032.44982541
TCGGAAG96100.031.353653
CATCTCG95150.030.16883739
GATCGGA101000.029.662121
Produced by FastQC (version 0.11.5)