FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12652187
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAAAGATCTCGTATGC298660.2360540513667716TruSeq Adapter, Index 7 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG37700.056.92197446
CGTATGC45850.046.65358444
GTATGCC46550.046.177145
ATGCCGT49350.043.7680447
TCGTATG49450.043.25750443
TGCCGTC55850.038.67371748
ACGTCTG57200.038.11656615
CTCGTAT56950.037.68308342
GCACACG60000.036.51133311
GCCGTCT60550.035.7860149
CACACGT62800.034.93929712
ACACGTC63850.034.30978413
CACGTCT72300.030.25133914
CGTCTGA73100.029.97039416
TCTCGTA77600.027.78987941
AGCACAC81900.027.51734510
GAGCACA87700.026.016629
ATCTCGT91650.023.56740640
GATCTCG92200.023.35069539
CAGTCAC94550.023.32302527
Produced by FastQC (version 0.11.5)