FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11977158
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT1570061.3108785907307894TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG169000.065.35223448
CGTATGC175750.063.18325446
GTATGCC177300.062.8070247
ATGCCGT175000.062.52834749
TCGTATG180250.061.4727345
TGCCGTC180850.060.09700450
ACGTCTG208100.059.68556215
CTCGTAT189100.059.2444944
GCACACG211100.058.8999811
GCCGTCT182700.057.89658751
CACACGT215600.057.76825312
ACACGTC217150.057.2753313
GTCAGAA219100.056.31648336
ATGTCAG219250.056.11408634
CACGTCT222550.055.87056414
ACATGTC221000.055.7817732
CGTCTGA222800.055.77971616
GTCACAT226450.054.51814329
TGTCAGA227050.054.3280535
TCTCGTA207100.054.07792743
Produced by FastQC (version 0.11.5)