FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ge-0_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13293133
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18050.038.40032246
GTCACGC25300.027.94731929
CGTATGC25500.027.73334144
GTATGCC28100.025.16622745
TCACGCC28000.025.12502530
ATGCCGT28450.024.85475547
TCGTATG32000.022.4281443
CACGCCA34350.021.19401431
TGCCGTC34650.020.60933748
TATCTCG37400.019.1894239
CTCGTAT40100.018.15975442
CGCCAAT40600.017.58648933
GCCGTCT40350.017.35024349
ACGCCAA43350.016.6323332
GCGGGCC3251.0004442E-1016.1533151
AGTCACG47150.015.59008828
GCGGGGT5950.015.2936141
ACGTCTG48600.015.05032315
GCCGGAT26400.013.5222831
GCGGACT11200.012.8120781
Produced by FastQC (version 0.11.5)