FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11834853
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT330280.2790740197618002TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG42300.054.28983748
CGTATGC45450.050.60762446
GTATGCC47500.048.64235347
ATGCCGT51500.044.99702549
TCGTATG53300.043.28808645
ACGTCTG58600.042.04341515
TATCTCG58000.040.32200241
TGCCGTC59250.039.05043850
CTCGTAT60450.038.80623244
GCACACG65800.037.33370211
CACACGT67050.036.89912412
GCCGTCT64200.036.0931551
ACACGTC68700.035.86008513
CACACTG66250.035.60956631
CGTCTGA77350.032.0789316
CACGTCT78750.031.37266214
TGATATA75900.031.13074536
ACACTGA78950.030.19157832
ACTGATA78700.030.0647334
TCTCGTA80550.029.2095543
Produced by FastQC (version 0.11.5)