FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15869247
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT2987491.8825656945159401TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG324250.066.3224848
CGTATGC331400.064.8940246
ATGCCGT334650.064.204849
GTATGCC335950.064.0660147
TCGTATG338750.063.2912345
TATCTCG342250.062.44785741
TCCGTAT345250.062.42621237
TGCCGTC345400.062.12291750
CGTATCT345200.061.9116539
CTCGTAT346250.061.84096544
ACGTCTG358800.061.62351615
CCGTATC351100.061.22886338
GTTCCGT353850.061.0335735
GCCGTCT351450.060.67446551
GCACACG365200.060.66510411
TTCCGTA358100.060.31186736
CACACGT367700.060.22427712
AGTTCCG362200.059.7108634
ACACGTC371500.059.60769313
CGTCTGA376800.058.69085316
Produced by FastQC (version 0.11.5)