FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Ct-1_LN_R1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13403967
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1070350.7985322554136398TruSeq Adapter, Index 5 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG135950.063.93697746
CGTATGC142650.060.9619444
ATGCCGT143850.060.083447
GTATGCC145800.059.81204245
TCGTATG147600.058.44332543
TGCCGTC150600.057.11084448
CTCGTAT140550.055.9692642
GCCGTCT156950.054.57391749
ACGTCTG165650.054.42383615
GCACACG174350.052.02636311
CACAGTG171800.051.64636233
CACACGT175950.051.47389212
ACACGTC177250.051.0172113
ACACAGT175200.050.56418232
CACACAG176100.050.50376531
CACGTCT181650.049.7045714
CGTCTGA182250.049.6022416
TCTCGTA165650.047.55071341
AGCACAC195900.046.46397410
TCACACA192050.046.43589830
Produced by FastQC (version 0.11.5)