FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12280203
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC210370.17130824303148734TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG29700.049.74018546
CGTATGC35100.042.19033444
GTATGCC37450.039.5420745
ATGCCGT39000.037.78853247
TCGTATG41200.035.94370343
TGCCGTC41700.035.59305648
ACGTCTG47050.032.43095815
GCACACG47550.032.38249211
CTCGTAT46700.031.4847142
GCCGTCT47900.031.35052949
ACGGTAG50800.029.42086232
CACACGT53200.029.14083312
AGTCACG52700.028.55998228
ACACGTC54950.028.33992213
GTCACGG53450.028.2247229
GGTAGCA57000.026.40416534
CACGTCT64050.023.98566414
CGTCTGA64100.023.80549616
AGCACAC69000.023.12724510
CGGTAGC65300.023.102433
Produced by FastQC (version 0.11.5)