FastQCFastQC Report
Wed 11 Jul 2018
HVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVF5GBGX5_n01_NutriNet.AtRep.Col-0_LN_R2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11781102
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAACAATCTCGTAT1325511.1251154603363929TruSeq Adapter, Index 13 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG147650.065.4839948
CGTATGC154250.062.75283446
GTATGCC154500.062.6494347
ATGCCGT156950.061.71241449
TCGTATG160400.060.39197545
TGCCGTC163250.059.3507950
CTCGTAT163950.058.85117744
GCCGTCT169600.057.06550651
ACGTCTG176600.055.40859215
GCACACG181650.054.1147511
CACACGT182050.053.97685612
TCTCGTA180200.053.46663743
ACACGTC184700.053.14512613
CACAGTC184000.052.75057631
ACAGTCA187550.051.92050632
CACGTCT191000.051.44757514
CGTCTGA191600.051.1636116
AATCTCG192350.050.14161741
GTCACAG195500.049.82896829
ATCTCGT194250.049.5985842
Produced by FastQC (version 0.11.5)